Department's employeess

Prof. Andrzej Kozik, PhD, DSc

Head of the Department and the Laboratory of Physical Biochemistry

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Room: A104
Phone: 012 6646525
 
Research summary: 
 
Modifications of the structure and functions of proteins during the inflammation. Macromolecular interactions in the kinin-generating systems and their modifications in the inflammatory foci. Biochemistry of oxidative stress. Biochemistry of vitamins and coenzymes. 
 
Most important scientific achievements:
 
• Characteristics of functions as well as the mechanism of protein-ligand interactions in the plant thiamin-binding proteins.
• Characteristics of the cellular mechanisms of activation of proinflammatory peptides - kinins.
• Hypothesis concerning modulation of kinins generation in inflammatory foci by reactive oxygen forms.
 
Selected publications:
  1. Imamura T., Tanase S., Szmyd G., Kozik A., Travis J., Potempa J. (2005) Induction of vascular leakage through release of bradykinin and a novel kinin by cysteine proteinases from Staphylococcus aureus. J. Exp. Med. 201, 1669-1676 
  2. Rapala-Kozik M., Olczak M., Ostrowska K., Starosta A., Kozik A. (2007) Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays: cDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methyl (phosphate) kinase and thiamine monophosphate synthase activities. Biochem. J. 408, 149-159 
  3. Barbasz A., Guevara-Lora I., Rapala-Kozik M., Kozik A. (2008) Kininogen binding to the surfaces of macrophages. Int. Immunopharmacol. 8, 211-216 
  4. Rapala-Kozik M., Karkowska J., Jacher A., Golda A., Barbasz A., Guevara-Lora I., Kozik A. (2008) Kininogen adsorption to the cell surface of Candida spp. Int. Immunopharmacol. 8, 237-241 
  5. Barbasz A., Kozik A. (2009) The assembly and activation of kinin-forming systems on the surface of human U-937 macrophage-like cells. Biol. Chem 390, 269-275 
  6. Guevara-Lora I., Florkowska M., Kozik A. (2009) Bradykinin-related peptides up-regulate the expression of kinin B1 and B2 receptor genes in human promonocytic cell line U937. Acta Biochim. Polon. 56, 515-522 
  7. Karkowska-Kuleta J., Kozik A., Rapala-Kozik M. (2010) Binding and activation of human plasma kinin-forming system on the cell walls of Candida albicans and Candida tropicalis. Biol. Chem. 391, 97-103 
  8. Rapala-Kozik M., Karkowska-Kuleta J., Ryzanowska A., Golda A., Barbasz A., Faussner A., Kozik A. (2010) Degradation of human kininogens with the release of kinin peptides by extracellular proteinases of Candida spp. Biol. Chem. 391, 823-830 
  9. Kozik A., Golda A., Mak P., Suder P., Silberring J., Barbasz A., Rapala-Kozik M.(2011) Myeloperoxidase-catalyzed oxidative inactivation of human kininogens: the impairment of kinin-precursor and prekallikrein-binding functions. Biol. Chem. 392, 263-274 
  10. Rapala-Kozik M., Bras G., Chruscicka B., Karkowska-Kuleta J., Sroka A., Herwald H., Nguyen K-A., Eick S., Potempa J., Kozik A. (2011) Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms. Infection and Immunity 79, 797-805  
Research grants:
  1. KBN No 6 P04C 088 12, „Structural and chemical properties of microenvironments of thiamine (vitamin B1) in the complexes with soluble binding proteins (transporters or enzymes) from bacteria, higher plants and vertebrates", 1997 – 1999, principal investigator
  2. KBN No 6 P04C 039 19, „Metabolism of thiamine (vitamin B1) and thiamine-binding proteins during plant seed development and germination" (principal investigator: dr Maria Rąpała-Kozik), 2000 – 2003, major contributor 
  3. KBN No 6 P04A 023 19, „Effects of neutrophil-derived agents (reactive oxygen forms and elastase) on the kinin-releasing potential of kininogen-kallikrein system", 2000 – 2003, principal investigator
  4. KBN No PBZ – 039/P04/02, „Comparison on the transcryptome and proteome levels of the expression of genes involved in the inflammatory responses of selected cell types", (principal investigator: prof. Aleksander Koj), 2001 – 2004, contributor 
  5. MNIiI No 2P04C 035 29, „ Binding and activation of kinin-generating systems on the cell surfaces", 2005 - 2008, principal investigator
  6. MNiSW No N N303 572538, „Characterization of the interactions between plasma kinin-generating proteins and the cells of Candida albicans yeast", 2010-2013, principal investigator 

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Prof. Adam Dubin, PhD, DSc

Professor Emeritus, former head of the Department

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Research summary:
 
Proteinase inhibitors play a key role in the maintenance of homeostasis by inhibition of their target enzymes. Therefore, the knowledge of enzyme-inhibitor system and kinetic parameters of their interaction is very important not only from the basic point of view. The earlier work was focused on purification and characterisation of leukocyte elastases and some major blood and tissue target inhibitors. Furthermore, the in vitro interaction between proteinases and their natural protein inhibitors as well as the amino acid sequence around the reactive centre of several serine proteinase inhibitors (serpins) has been established. The primary structure of tissue serpin inhibitor HLEI has been determined by the chemical and molecular cloning methods [1, 2]. Present studies concentrate on characterisation of bacterial cysteine proteinases and their inhibitors [5, 9]. Three-dimensional structure of newly discovered class of cysteine proteinase inhibitors - staphostatins have been solved by crystallography or NMR techniques [6, 7]. Currently we investigate DNA sequence of S. aureus plasmid pCH-91, their bioinformatics analysis and characteristics of protein coding in plasmid – see Dr. Benedict Wladyka's page. Additional work is oriented toward antibacterial peptides (peptide antibiotics) called hemocidins, produced during proteolytic digestion of haemoglobin - for more details see Dr. Pawel Mak's page. 
 
Selected publications:
  1. Dubin A., Travis J., Enghild J.J., Potempa J.: Equine Leukocyte Elastase Inhibitor. Primary structure and identification as a thymosin binding protein. J. Biol. Chem. 267 (1992) 6576–6583.
  2. Kordula T., Dubin A., Schooltink H., Koj A., Heinrich P.C., Rose-John S.: Molecular cloning and expression of an intracellular serpin: an elastase inhibitor from horse leucocytes. Biochem. J. 293 (1993) 187–193.
  3. Dubin A., Potempa J., Travis J.: Structural and functional characterization of elastases from horse neutrophils. Biochem. J. 300 (1994) 401–406.
  4. Imamura T., Dubin A., Tanaka, R., Travis J.: Induction of vascular permeability enhancement by human tryptase. Dependence of activation of prokallikrein and direct release of bradykinin from kininogens. Laboratory Investigation 74 (1996) 861-870.
  5. Rzychon M., Sabat A., Kosowska K., Potempa J., Dubin A.:Staphostatin: an expanding new group of proteinase inhibitors with a unique specificity for the regulation of staphopains, Staphylococcus spp. cysteine Proteinases. Mol. Microb. 49(2003)1051-1066.
  6. Filipek R., Rzychoń M., Oleksy A., Gruca M., Dubin A., Potempa J., Bochtler M.: The staphostatin-staphopain complex: a forward binding inhibitor in complex with target cysteine protease. J. Biol. Chem. 42(2003)40959-40966.
  7. Dubin G., Krajewski M., Popowicz G., Stec-Niemczyk J., Bochtler M., Potempa J., Dubin A., Holak T.A.: Solution structure of staphostain A from Staphylococcus aureus confirms the discovery of the novel class of cysteine proteinase inhibitors. Biochemistry 42(2003) 13449-13456.
  8. Władyka B., Puzia K., Dubin A.: Efficient co-expression of recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli. Biochem. J.385(2005)181-187.
  9. Popowicz G.M., Dubin, G., Steć-Niemczyk, J., Czarny, A., Dubin, A., Potempa, J., Holak, T.A.: functional and structural characterization of Spl proteases from Staphylococcus aureus. J. Mol. Biol. 358(2006)270-9.
  10. Bonar E., Dubin A., Bierczynska-Krzysiek A,. Noga M., Silberring J., Stalińska K., Koj A.: Identification of major cellular proteins synthesized in response to interleukin-1 and interleukin-6 in human Hepatoma HepG2 cells. Cytokine 33(2006)111-7.
Grants:
  1. Grant KBN: (no 4 1618 91 01) "Structure and function of elastase inhibitor", (1992-1994)
  2. Grant KBN: (no 4 P207 038 07) "Characteristic and application of research model of lung emphysema", (1995-1997).
  3. Grant BW-UJ: "Characteristics of antibacterial peptides from hamster PMN's", (96/97). 
  4. Grant KBN: (no 6 P04B 009 14: "Hemocidins: antibacterial peptides obtaining from heme containing proteins", (1998-2000).
  5. Grant KBN: (no 6 P04A 083 20) "New endopeptidase Staphylococcus aureus operon – analysis and regulation". (2001-2003).
  6. Grant KBN: (no 2 P04A 040 26) "Staphostatyn B: New type of cysteine proteinase inhibitor". (2004-2005).
  7. Grant KBN: (no 2 PO4A 001 27) "Metalloprotease operon of Staphylococcus aureus: analysis and regulation", (2004-2007).
  8. Grant MNiSzW: (no 2 P04A 024 30) „Staphopain C operon of Staphylococcus aureus: analysis and characteristic of coding proteins" (2006-2007).
  9. Grant MNiSzW: (no N N 302 1307 34) „Plasmid pCH-91 as virulence factors carrier and possible tool for efficient production of recombinant proteins", (2008-2011).

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Paweł Mak, PhD, DSc

Associate Professor, head of the department's Laboratory of Enzymology and Protein Chemistry

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Room: A119
Phone: 12 6646506
Fax: 12 6646915
 
Research summary: 
 
Peptides and proteins biochemistry (techniques of purification, determination and sequencing), isolation and characterization of antibacterial peptides and proteins.
 
Selected publications:
  1. Knutelski, S.; Awad, M.; Łukasz, N.; Bukowski, M.; Śmiałek, J.; Suder, P.; Dubin, G.; Mak, P. Isolation, Identification, and Bioinformatic Analysis of Antibacterial Proteins and Peptides from Immunized Hemolymph of Red Palm Weevil Rhynchophorus ferrugineus. Biomolecules 2021, 11, 83. https://doi.org/10.3390/biom11010083.
  2. Milewska A, Falkowski K, Kulczycka M, Bielecka E, Naskalska A, Mak P, Lesner A, Ochman M, Urlik M, Diamandis E, Prassas I, Potempa J, Kantyka T, Pyrc K. (2020) Kallikrein 13 serves as a priming protease during infection by the human coronavirus HKU1. Sci. Signal. 13, eaba9902. DOI: 10.1126/scisignal.aba9902.
  3. Sowa-Jasiłek A, Zdybicka-Barabas A, Stączek S, Pawlikowska-Pawlęga B, Grygorczuk-Płaneta K, Skrzypiec K, Gruszecki WI, Mak P, Cytryńska M (2020) Antifungal Activity of Anionic Defense Peptides: Insight into the Action of Galleria mellonella Anionic Peptide 2.. Int J Mol Sci. 21,1912. doi: 10.3390/ijms21061912.
  4. Koliński M, Kmiecik S, Dec R, Piejko M, Mak P, Dzwolak W (2020) Docking interactions determine early cleavage events in insulin proteolysis by pepsin: Experiment and simulation.. Int J Biol Macromol. 149, 1151-1160. doi: 10.1016/j.ijbiomac.2020.01.253.
  5. Palusińska-Szysz, M.; Zdybicka-Barabas, A.; Luchowski, R.; Reszczyńska, E.; Śmiałek, J.; Mak, P.; Gruszecki, W.I.; Cytryńska, M. (2020) Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III. Int. J. Mol. Sci. 21, 5818.
  6. Nowakowski M, Jaremko Ł, Wladyka B, Dubin G, Ejchart A, Mak P. Spatial attributes of the four-helix bundle group of bacteriocins - The high-resolution structure of BacSp222 in solution (2018) Int. J. Biol. Macromol. 107B, 2715-2724. doi: 10.1016/j.ijbiomac.2017.10.158.
  7. Pieta P, Majewska M, Su Z, Grossutti M, Wladyka B, Piejko M, Lipkowski J, Mak P. (2016) Physicochemical Studies on Orientation and Conformation of a New Bacteriocin BacSp222 in a Planar Phospholipid Bilayer. Langmuir 32, 5653-62, doi: 10.1021/acs.langmuir.5b04741.
  8. Palusińska-Szysz M, Zdybicka-Barabas A, Reszczyńska E, Luchowski R, Kania M, Gisch N, Waldow F, Mak P, Danikiewicz W, Gruszecki WI, Cytryńska M. (2016) The lipid composition of Legionella dumoffii membrane modulates the interaction with Galleria mellonella apolipophorin III. Biochim. Biophys. Acta. 1861, 617-29, doi: 10.1016/j.bbalip.2016.04.011
  9. Sowa-Jasiłek A, Zdybicka-Barabas A, Staczek S, Wydrych J, Skrzypiec K, Mak P, Deryło K, Tchórzewski M, Cytrynska M. (2016) Galleria mellonella lysozyme induces apoptotic changes in Candida albicans cells. Mic. Res. 193, 121–131, http://dx.doi.org/10.1016/j.micres.2016.10.003.
  10. Klaus T, Stalińska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J. (2018) Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Sci Rep. 8: 519. doi: 10.1038/s41598-017-19003
  11. Walentowicz-Sadlecka M, Dziobek K, Grabiec M, Sadlecki P, Walentowicz P, Mak P, Szymankiewicz M, Kwinta P, Dutsch-Wicherek M. (2019)The analysis of human leukocyte antigen-G level in patients with endometrial cancer by Western blot technique. Am J Reprod Immunol. 81, e13070. doi: 10.1111/aji.13070.
  12. Wladyka B, Piejko M, Bzowska M, Pieta P, Krzysik M, Mazurek Ł, Guevara-Lora I, Bukowski M, Sabat AJ, Friedrich AW, Bonar E, Międzobrodzki J, Dubin A, Mak P. (2015) A peptide factor secreted by Staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors. Sci Rep. 5:14569. doi: 10.1038/srep14569.
  13. Pietrzyk AJ, Bujacz A, Mak P, Potempa B, Niedziela T. (2015) Structural studies of Helix aspersa agglutinin complexed with GalNAc: A lectin that serves as a diagnostic tool. Int. J. Biol. Macromol. 81:1059-1068.

Research grants:

  1. Head of the project: "Studies on regulation of biosynthesis and on proinflammatory properties of peptide bacteriocin BacSp222 isoforms", NCN OPUS 16 grant No UMO-2018/31/B/NZ3/01226, years of realization 2019-2022.
  2. Head of the project: „Badania nad bakteriocynami i czynnikami wirulencji odzwierzęcego szczepu Staphylococcus pseudintermedius 222”, NCN grant No UMO-2013/11/B/NZ6/00409, realization: 2014-2018.
  3. Head of the project: "Badania nad modulacyjnym mechanizmem działania bakteriocyny peptydowej BacSp222 wobec komórek eukariotycznych", MNiSW / KNOW grant No 35p/9/2015, realization: 2016-2017
  4. Head of the project: „Rola peptydaz aspartylowych Candida Spp. w generowaniu hemocydyn", No 2011/01/B/NZ6/00277, end planned in 2013.
  5. Staff member of the project: „Apolipoforyna III Galleria mellonella - bialko o wielu funkcjach", No N N303 58 02 39, head: M. Cytryńska, end in 2012.
  6. Staff member of the project: MNiSw No N N303 572538, 2010-2013. „Charakterystyka oddziaływania białek osoczowego układu generacji kinin (układu kontaktu) z komórkami drożdży Candida albicans. Head: A. Kozik.
  7. Staff member of the project: „Ocena zmian aktywności komórek układu immunologicznego w endometrium podczas fizjologicznego cyklu miesiączkowego, w doczesnej w warunkach fizjologicznych i w stanach patologicznych wikłających przebieg ciąży, oraz w ogniskach endometriozy i raku jajnika", No N N407 0888 35, head: A. Basta, end in 2011.
  8. Staff member of the project: „Mechanizmy odpowiedzi humoralnej Galleria mellonella w zakażeniach bakteryjnych i grzybowych – analiza profilu oraz roli peptydów odpornościowych hemolimfy", No N N303 3622 35, head: M. Cytryńska, end in 2010.
  9. Head of the project: „Znaczenie hemocydyn w wybranych niespecyficznych mechanizmach obrony organizmu ludzkiego przed infekcjami", No N N401 2296 33, end in 2008.
  10. Staff member of the project: „Analiza molekularnych zmian w trakcie przemiany doczesnowej endometrium warunkujących prawidłowy przebieg ciąży i porodu", grant No N406 012 31/0201, ended in 2008.
  11. Staff member of the project: „Identyfikacja oraz ocena przeciwbakteryjnych i przeciwgrzybowych właściwości peptydów hemolimfy Galleria mellonella", grant No 2 PO4C 054 26, head: M. Cytryńska, ended in 2006.
  12. Head of the project: „Naturalne peptydy antybakteryjne uwalniane z białek wiążących hem", grant No 2 PO4B 023 27, ended in 2006.

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Ibeth Guevara-Lora, PhD, DSc

Associate Professor

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Room: A10
Phone: 012 6646544, 6646527
 
Research summary:
 
Expression of genes and proteins involved in the regulation of inflammation, especially in relation to the kinin generation system. Biochemical applications of biologically active substance immobilized in nanoparticles.
 
Selected publications:
  1. Guevara I., Iwanejko J., Dembińska-Kieć A., Pankiewicz J., Wanat A., Polus A., Gołąbek I., Bartuś S., Malczewska-Malec M., Szczudlik A. (1998) Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin. Chim. Acta 274, 177-188. 
  2. Dulak J., Józkowicz A., Dembiñska-Kieć A., Guevara I., Zdzienicka A., Żmudzinska-Grochot D., Florek I., Wójtowicz A., Szuba A., Cooke JP. (2000) Nitric oxide induces the synthesis of vascular endothelial growth factor by rat vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 20, 659-666.
  3. Józkowicz A., Cooke J.P., Guevara I., Huk I., Funovics P., Pachinger O., Weidinger F., Dulak J. (2001) Genetic augmentation of nitric oxide synthase increases the vascular generation of VEGF. Cardiovasc. Res. 51, 773-783.
  4. Tomaszewska A., Guevara I., Wilczok T., Dembińska-Kieć A. (2001) 17-estradiol- and lipopolysaccharide-induced changes in nitric oxide, tumor necrosis factor-a and vascular endothelial growth factor release from RAW 264.7 macrophages. Gynecol. Obstet. Invest. 56, 152-159.
  5. Duliński R., Suder P., Guevara-Lora I., Rąpała Kozik M., Potempa J., Silberring J., Imamura T., Travis J., Kozik A. (2003) Attenuated kinin release from human neutrophil elastase-pretreated kininogens by tissue and plasma kallikreins. Biol. Chem. 384, 929-937.
  6. Barbasz A., Guevara-Lora I., Rąpała-Kozik M., Kozik A. (2008) Kininogen binding to the surfaces of macrophages. Int. Immunopharmacol. 8, 211-216.
  7. Rąpała-Kozik M., Karkowska J., Jacher A., Gołda A., Barbasz A., Guevara-Lora I., Kozik A. (2008) Kininogen adsorption to the cell surface of Candida spp., Int. Immunopharmacol. 8, 237-241.
  8. Guevara-Lora I., Czosnek C., Smycz A., Janik J.F, Kozik A. (2008) SiC nanoparticles as potential carriers for biologically active substances. J. Phys., Conference Series 146, 012-022
  9. Guevara-Lora I., Florkowska M., Kozik A. (2009) Bradykinin-related peptides up-regulate the expression of kinin B1 and B2 receptor genes in human promonocytic cell line U937. Acta Biochim. Polon. 56, 515-522 
  10. Taha H., Skrzypek K., Guevara I., Nigisch A., Mustafa S., Grochot-Przeczek A., Ferdek P., Was H., Kotlinowski J., Kozakowska M., Balcerczyk A., Muchova L., Vitek L., Weigel G., Dulak J., Jozkowicz A. (2010) Role of heme oxygenase-1 in human endothelial cells: lesson from the promoter allelic variants. Arterioscler. Thromb. Vasc. Biol. 30, 1634-1641.
Research grants:
  1. Principal Investigator, grant from the Minister of Science and Higher Education "The effect of selected products derived from the contact system activation on the adhesion of cells involved in coagulation blood during inflammation.(2006-2009).
  2. Senior staff member in the grant from the Committee of Scientific Research "Role of nitric oxide and vascular endothelial growth factor in restenosis – the effect of experimental gene transfer. Principal Investigator: Prof. dr hab. Józef Dulak (1998–2001).
  3. Role of peroxisome proliferator activated receptors-(PPAR) in angiogenesis. Principal investigator: Dr hab. Alicja Józkowicz (1999-2002).
  4. Correlation between the mutations of selected genes and the risk factors related to obesity and insulin resistance in selected families from the South of Poland. Principal investigator: Prof. dr hab. Aldona Dembińska-Kieć (1999–2002).
  5. The role of nitric oxide and heme oxygenase in angiogenic activity of vascular endothelial growth factor VEGF. Principal Investigator: Prof. dr hab. Józef Dulak. (2002-2005)
  6. Binding and activation of kinin-generating systems on the cell surface. Principal Investigator: Prof. dr hab. Andrzej Kozik (2005-2008).

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Benedykt Władyka, PhD, DSc

Associate Professor

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Room: A124
Phone: 12 6646511
E-mail: benedykt.wladyka@uj.edu.pl

Scientific interests:
Bacterial toxin-antitoxin systems. Proteases and their inhibitors. Staphylococcal virulence factors

Selected publications:

  1. Bonar, E.A., Bukowski, M., Hydzik, M., Jankowska, U., Kedracka-Krok, S., Groborz, M., Dubin, G., Akkerboom, V., Miedzobrodzki, J., Sabat, A.J., Friedrich, A.W., Władyka, B. Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains (2018) Frontiers in Cellular and Infection Microbiology, 8 art. no. 313. DOI: 10.3389/fcimb.2018.00313
  2. Władyka, B., Bonar, E. Application of Staphylococci in the Food Industry and Biotechnology (2018) Pet-to-Man Travelling Staphylococci: A World in Progress, pp. 281-291. DOI: 10.1016/B978-0-12-813547-1.00021-2
  3. Bukowski, M., Hyz, K., Janczak, M., Hydzik, M., Dubin, G., Władyka, B. Identification of novel mazEF/pemIK family toxin-antitoxin loci and their distribution in the Staphylococcus genus (2017) Scientific Reports, 7 (1), art. no. 13462, . DOI: 10.1038/s41598-017-13857-4
  4. Bonar, E., Wojcik, I., Jankowska, U., Kedracka-Krok, S., Bukowski, M., Polakowska, K., Lis, M.W., Kosecka-Strojek, M., Sabat, A.J., Dubin, G., Friedrich, A.W., Miedzobrodzki, J., Dubin, A., Władyka, B. Identification of secreted exoproteome fingerprints of highly-virulent and non-virulent Staphylococcus aureus strains (2016) Frontiers in Cellular and Infection Microbiology, 6 (MAY), art. no. 51, . DOI: 10.3389/fcimb.2016.00051
  5. Władyka, B., Piejko, M., Bzowska, M., Pieta, P., Krzysik, M., Mazurek, Ł., Guevara-Lora, I., Bukowski, M., Sabat, A.J., Friedrich, A.W., Bonar, E., Miȩdzobrodzki, J., Dubin, A., Mak, P. A peptide factor secreted by Staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors (2015) Scientific Reports, 5, art. no. 14569, . DOI: 10.1038/srep14569
  6. Bonar, E., Wójcik, I., Władyka, B. Proteomics in studies of Staphylococcus aureus virulence (2015) Acta Biochimica Polonica, 62 (3), pp. 367-381. DOI: 10.18388/abp.2015_1083
  7. Władyka, B., Wielebska, K., Wloka, M., Bochenska, O., Dubin, G., Dubin, A., Mak, P. Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91 (2013) Applied Microbiology and Biotechnology, 97 (16), pp. 7229-7239. DOI: 10.1007/s00253-012-4578-y
  8. Bukowski, M., Lyzen, R., Helbin, W.M., Bonar, E., Szalewska-Palasz, A., Wegrzyn, G., Dubin, G., Dubin, A., Władyka, B. A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIK Sa (2013) Nature Communications, 4, art. no. 2012, . DOI: 10.1038/ncomms3012
  9. Władyka, B., Kozik, A.J., Bukowski, M., Rojowska, A., Kantyka, T., Dubin, G., Dubin, A. α1-Antichymotrypsin inactivates staphylococcal cysteine protease in cross-class inhibition (2011) Biochimie, 93 (5), pp. 948-953. DOI: 10.1016/j.biochi.2011.01.014
  10. Władyka, B., Dubin, G., Dubin, A. Activation mechanism of thiol protease precursor from broiler chicken specific Staphylococcus aureus strain CH-91 (2011) Veterinary Microbiology, 147 (1-2), pp. 195-199. DOI: 10.1016/j.vetmic.2010.06.002
  11. Władyka, B., Puzia, K., Dubin, A. Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli (2005) Biochemical Journal, 385 (1), pp. 181-187. DOI: 10.1042/BJ20040958


Research grants:

  1. MO-2017/25/B/NZ6/01056 – “ Studies of the role of toxin-antitoxin systems in antibiotics resistance in staphylococci”, granted by National Science Centre (NCN) in frame of „Opus 13” (2018-2021), – PI
  2. UMO-2014/13/B/NZ1/00043 – „Toxin-antitoxin systems as regulators of gene expression in Staphylococcus”, granted by National Science Centre (NCN) in frame of „Opus 7” (2015-2019), – PI
  3. UMO-2012/07/D/NZ2/04282 – „Identification and studies of factors involved in staphylococcal virulence using genomics and proteomics”, granted by National Science Centre (NCN) in frame „Sonata 4” (2013-2016), – PI
  4. N N303 813340 – “Identification and characteristics of factors involved in host specific colonization and virulence of Staphylococcus aureus”, granted by Ministry of Science and Higher Education (MNiSW) (2011-2014), – PI
  5. IP 2010 046770 – “Studies of molecular bases of virulence of bacteria from Staphylococcus genus”, granted by Ministry of Science and Higher Education (MNiSW) in frame of “Iuventus Plus” (2011), – PI

 

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Emilia Bonar, PhD Eng.

Adiunct

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Room: A118
Phone: 12 6646511
 

Research interests:

Staphylococcal toxin-antitoxin systems. Staphylococcal virulence factors. Proteomics techniques in studying cellular responses to environmental factors and in identifying factors involved in virulence.

Collaboration:

  1. High Resolution Mass Spectrometry Laboratory, Faculty of Chemistry of the Jagiellonian University, prof. dr hab. Wojciech Piekoszewski, MA Joanna Kasprzyk
  2. Pedagogical University of Cracow,  Institute of Biology, Department of Cell Biology and Genetics, dr hab. Maria Wędzony, dr Gabriela Gołębiowska-Pikania
  3. Jagiellonian Centre for Experimental Therapeutics (JCET), Laboratory of Analytics and Pharmacokinetics, dr hab. Maria Walczak, dr Anna Kurpińska

Selected publications:

  1. Bonar E, Chlebicka K, Dubin G, Wladyka B, Identification of Staphylococcus aureus virulence factors using proteomic approaches Application of Two-dimensional Differential in Gel Electrophoresis in Identification of Factors Responsible for Virulence in Staphylococcus aureus  (2020) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols, Springer. Editor: Ji, Yinduo (Ed.) Methods Mol Biol. 2020;2069:139-154. doi: 10.1007/978-1-4939-9849-4_11.
  2. Gołębiowska G., Bonar E., Emami K., Wędzony M. Cold-modulated small proteins abundance in winter triticale (x Triticosecale, Wittm.) seedlings tolerant to the pink snow mould (Microdochium nivale, Samuels & Hallett) infection. Acta Biochim Pol. (2019) Sep 10;66(3):343-350. doi: 10.18388/abp.2019_2804.
  3. A. Kurpinska, J. Suraj, E. Bonar, A. Zakrzewska, M. Stojak, M. Sternak, A. Jasztal, M. Walczak (2019). Proteomic characterization of early lung response to breast cancer metastasis in mice. Exp Mol Pathol. 2019 Feb 11. pii: S0014-4800(18)30482-9. doi: 10.1016/j.yexmp.2019.02.001
  4. E. Bonar, M.Bukowski, M.Hydzik, U.Jankowska, S.Kedracka-Krok, G.Dubin, J. Miedzobrodzki, A.J.Sabat, A.W.Friedrich, B.Wladyka (2018). Joint genomic and proteomic analysis identifies meta-traits characteristic for virulent and non-virulent S. aureus strains. Front Cell Infect Microbiol. 2018 Sep 6;8:313. doi: 10.3389/fcimb.2018.00313. eCollection 2018.
  5. E. Bonar, J.Międzobrodzki, B.Władyka (2018) Chapter7. The Staphylococcal Coagulases. pp. 95-102; Pet-to-man Travelling Staphylococci: A World in Progress edited by Vincenzo Savii, ISBN: 978-0-12-813547-1, Document Type: Book Chapter, Publisher: Elsevier Inc. doi.org/10.1016/B978-0-12-813547-1.00007-8  
  6. B. Władyka, E. Bonar (2018) Chapter 21. Application of Staphylococci in the Food Industry and Biotechnology. pp. 281-291; Pet-to-Man Travelling Staphylococci: A World in Progress edited by Vincenzo Savii, ISBN: 978-0-12-813547-1, Document Type: Book Chapter, Publisher: Elsevier Inc. doi.org/10.1016/B978-0-12-813547-1.00021-2
  7. Bonar E., Wojcik I., Jankowska U., Kedracka-Krok S., Bukowski M., Polakowska K., Lis M.W., Kosecka-Strojek M., Sabat A.J., Dubin G., Friedrich A.W., Miedzobrodzki J., Dubin A. and Wladyka B. (2016) Identification of secreted exoproteome fingerprints of highly-virulent and non-virulent Staphylococcus aureus strains. Front. Cell. Infect. Microbiol. 6:51. doi: 10.3389/fcimb.2016.00051.
  8. Wladyka B., Piejko M., Bzowska M., Pieta P., Krzysik M., Mazurek Ł., Guevara-Lora I., Bukowski M., Sabat AJ., Friedrich AW., Bonar E., Międzobrodzki J., Dubin A., Mak P. (2015) A peptide factor secreted by Staphylococcuspseudintermedius exhibits properties of both bacteriocins and virulence factors. Sci. Rep. 5, 14569; doi: 10.1038/srep14569
  9. Bonar E., Wójcik I., Władyka B. (2015) Proteomics in studies of Staphylococcus aureus virulence. Acta Biochim Pol. 2015;62(3):367-81. doi: 10.18388/abp.2015_1083
  10. Bukowski M, Lyzen R, Helbin WM, Bonar E, Szalewska-Palasz A, Wegrzyn G, Dubin G, Dubin A, Wladyka B. (2013). A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIKSa. Nat Commun. Jun 18;4:2012. doi: 10.1038/ncomms3012.

Research grants:

  1. Head of the project 2018/02/X/NZ2/03551, Miniatura 2; „Transcriptomic and proteomic analysis of mutation effects causing overproduction of PVL toxin in Staphylococcus aureus”.
  2. Head of the project: "Identification of substrate specificity and the role of toxins of toxin-antitoxin systems using transcriptomic and proteomics techniques". IMPULS Program co-financing scientific activity of FBBB employees. KNOW, CELL-MOL-TECH Consortium.
  3. Staff member of the project: 2017/25/B/NZ6/01056; Studies of the role of toxin-antitoxin systems in antibiotics resistance in staphylococci”, granted by National Science Centre (NCN) in frame of „Opus 13” (2018-2021) head: dr hab.  B. Władyka.
  4. Staff member of the project: UMO-2014/13/B/NZ1/00043 – „Toxin-antitoxin systems as regulators of gene expression in Staphylococcus”, granted by National Science Centre (NCN) in frame of „Opus 7” (2015-2019) head: dr hab.  B. Władyka.
  5. Staff member of the project: UMO-2012/07/D/NZ2/04282 – „Identification and studies of factors involved in staphylococcal virulence using genomics and proteomics”, granted by National Science Centre (NCN) in frame „Sonata 4” (2013-2016) head: dr hab.  B. Władyka.
  6. Staff member of the project: N N303 813340 – “Identification and characteristics of factors involved in host specific colonization and virulence of Staphylococcus aureus”, granted by Ministry of Science and Higher Education (MNiSW) (2011-2014) head: dr hab.  B. Władyka.

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Michał Bukowski, PhD

Adjunct

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Room: A125
Tel.: 12 664 6543, 12 664 6099
E-mail: m.bukowski@uj.edu.pl
 
Research summary:
 
Virulence mechanisms of Staphylococcus aureus in the context of host-patogen interactions. Staphylococci internalisation to eukaryotic cells, mechanisms of its regulation and regulatory systems determining the intra-endosomal survival. Class II toxin-antitoxin systems (TAS) and their role in gene expression regulation, global transcriptome changes and persister cells forming. Comparative genomics and high-throughput analysis of Next Generation Sequencing data, python and C++ programming in data mining & analysis.
 
Selected publications:
 
Original papers:
  1. Savini V, Kosecka K, Siegwart E, Marrollo R, Polilli E, Palmieri D, Fazii P, Carretto E, Międzobrodzki J, Bukowski M. (2015) Daptomycin-resistant Staphylococcus pettenkoferi of human origin. Acta Biochimica Polonica [Epub ahead of print].
  2. Wladyka B, Piejko M, Bzowska M, Pieta P, Krzysik M, Mazurek Ł, Guevara-Lora I, Bukowski M, Sabat AJ, Friedrich AW, Bonar E, Międzobrodzki J, Dubin A, Mak P. (2015) A peptide factor secreted by Staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors. Scientific Reports 5:14569.
  3. Janczak M, Wladyka B, Bukowski M, Gorecki A, Dubin G, Dubin A. (2015) A systematic investigation of the stability of green fluorescent protein fusion proteins. Acta Biochimica Polonica 62(3):407-11.
  4. Bukowski M, Polakowska K, Ilczyszyn WM, Sitarska A, Nytko K, Kosecka M, Miedzobrodzki J, Dubin A, Wladyka B. (2015) Species determination within Staphylococcus genus by extended PCR-restriction fragment length polymorphism of saoC gene. FEMS Microbiology Letters 362 (1): 1-11.
  5. Bukowski M, Lyzen R, Helbin WM, Bonar E, Szalewska-Palasz A, Wegrzyn G, Dubin G, Dubin A, Wladyka B. (2013) A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIK Sa . Nature Communications 4:2012.
  6. Wladyka B, Kozik AJ, Bukowski M, Rojowska A, Kantyka T, Dubin G, Dubin A. (2011) alpha(1)-Antichymotrypsin inactivates staphylococcal cysteine protease in cross-class inhibition. Biochimie 93(5): 948-53.
Review papers:
  1. Bukowski M, Rojowska A, Wladyka B. (2011) Prokaryotic toxin-antitoxin systems - the role in bacterial physiology and application in molecular biology. Acta Biochimica Polonica 58(1): 1-9.
  2. Bukowski M, Wladyka B, Dubin G. (2010) Exfoliative toxins of Staphylococcus aureus. Toxins 2(5): 1148-1165.
Research grants:
  1. Research Scientist in the project founded by the National Science Center (NCN, Poland) No. UMO-2012/07/D/NZ2/04282: Identification and characterisation of staphylococcal virulence factors by genomics and proteomics. Principal Investigator: dr Benedykt Władyka (2013-2016).
  2. Principal Investigator in the project founded by the National Science Center (NCN, Poland) No. UMO-2011/01/N/NZ1/01167: Functional analysis of the anisig-sig operon of Staphylococcus aureus (2011-2014).
  3. Research Scientist in the project founded by the Ministry of Science and Higher Education (MNiSW, Poland) No. N N303 813340: Identification and characterisation of factors related to host-specific colonisation and virulence of Staphylococcus aureus strains. Principal Investigator: dr Benedykt Władyka (2011-2014).
  4. Research Scientist in the project founded by the Ministry of Science and Higher Education (MNiSW, Poland) No. IP 2010 046770: Molecular basis of Staphylococcus aureus virulence. Principal Investigator: dr Benedykt Władyka (2010-2011).
  5. Research Scientist in the project founded by the Ministry of Science and Higher Education (MNiSW, Poland) No. N N302 130734: Staphylococcal pCH91 plasmid as a carrier of virulence factors genes and potential tool for recombinant protein production. Principal Investigator: prof. dr hab. Adam Dubin (2008-2011).

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Kinga Chlebicka, MSc

Assistant

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Kamila Kulig, MSc

Assistant

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Ewelina Wronowska, MSc

Biologist