- Isolation and biochemical characterisation of proteolytic enzymes and their natural protein inhibitors of bacterial origin.
- Protein expression in heterological bacterial system.
- Cloning, expression and purification of recombinant proteins.
- Protein folding processes.
- Antibacterial peptides: isolation, purification, sequencing and biochemical characterisation.
- Bacteriocins: isolation, purification, sequencing and biochemical characterisation.
- New techniques in protein and peptide chemistry.
- Studies on interaction of MDM2 and MDM4 proteins with p53 – in close cooperation with Dr. Tad Holak from Max-Planck Institute of Biochemistry in Munich.
- Identification and kinetic characterization of enzymes involved in plant thiamine biosynthesis.
- Structural characterization of thiamine biosynthesis enzymes with site-direct mutagenesis.
- Interaction of the contact system proteins, kininogen, prekallikrein and factor XII with the surface of selected Candida species.
- Kinin generation by secreted proteinases from Candida spp.
- Biochemical applications of biologically active substance immobilized in silicon carbide nanoparticles.
- Mechanism of kinin generation in tissue cell models.
- Expression of genes and proteins involved in the regulation of inflammation, especially in relation to the kinin generation system.
Subjects of MSc theses realized in the Department